Purification of PCR Products Using Bandstab PCR Method: Application to Short 16s-23s Intergenic Spacer Region of Lactobacillus Spp

ISSN: 0973-7510

E-ISSN: 2581-690X

Purification of PCR Products Using Bandstab PCR Method: Application to Short 16s-23s Intergenic Spacer Region of Lactobacillus Spp

Tanedjeu Sonfack Kemgang^1,2 , Huguette Gaelle Ngassa Mbenda³, Suman Kapila¹ and Rajeev Kapila¹

¹National Dairy Research Institute, Animal Biochemistry Division, 132001 Karnal, Haryana, India.

²University of Ngaoundere, National School of AgroIndustrial Sciences, Department of Food Science and Nutrition, PO Box 455, Ngaoundere, Adamaoua, Cameroon.

³National Institute of Malaria Research, Evolutionary Genomics and Bioinformatics,
110077 New Delhi, Delhi, India.

J Pure Appl Microbiol. 2015;9(3):2251-2254

Received: 10/05/2015 | Accepted: 03/07/2015 | Published: 30/09/2015

Abstract

In view to show a simple DNA purification step from agarose gel, we applied the DNA bandstab technique to the 16S-23S Intergenic Spacer Region (ISR) (a polymorphic region resulting in multiple bands after gel-electrophoresis) of selected Lactobacillus spp. The short ISR was successfully extracted by bandstab after numerous unsuccessful attempts of DNA cutting-elution techniques. The purification of band was further confirmed by the distinctive peaks observed in the DNA chromatograms generated after the sequencing procedure.

Keywords

Bandstab-PCR, Lactobacillus, 16S-23S intergenic spacer region, Sequencing

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