ISSN: 0973-7510

E-ISSN: 2581-690X

O. Emmanuel Nwafor
Department of Microbiology, Delta State University, Abraka, Nigeria.
J Pure Appl Microbiol. 2012;6(4):1803-1809
© The Author(s). 2012
Received: 12/06/2012 | Accepted: 30/07/2012 | Published: 31/12/2012
Abstract

Protein enrichment of wastes from Dioscorea rotundata, Dioscorea cayenensis and Dioscoreaalata was investigated by solid state fermentation  using mono and co-cultures of four amylolytic fungi. The wastes provided the sole carbon source while mineral elements were from (NH4)SO4, urea and KHPO4. Fermentation was allowed to progress for 5days. pH, moisture and protein contents were assessed on the first day and on the 5th day for the various yam varieties. Protein contents increased from the value of 3.82±0.29% (w/w) to values that ranged from 8.46±0.30% (w/w) from S. cerevisiae on D.rotundata to 10.10±0.50% (w/w) from Mucor sp on D.alata. Addition of booster doses of nitrogenous supplements resulted in further increases in protein yield. The use of co-cultures of the fungi showed a further increase in protein although not up to double for the values obtained from those where mono cultures were inoculated. There were decreases in pH values with increasing moisture contents. Results indicate the possibility of the use of the bioconverted wastes as feeds for poultry and other animals after appropriate toxicological studies.

Keywords

Protein, Enrichment, Mono, Co-cultures, Amylolytic fungi

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© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.