ISSN: 0973-7510
E-ISSN: 2581-690X
In the present study metagenomic library was created from soil sample collected from a hot water spring located in the district Chamoli, Uttarakhand, India. The genomic DNA was partially digested with Sau3AI and the restriction fragments were eluted from the agarose gel. The purified fragments were ligated with plasmid vector and transformed into E.coli cells. A metagenomic library of insert size approximately 1.6 kb was constructed. A total number of 60,000 clones were screened for secretion of industrial enzymes of which two were detected positive for amylase activity.
Metagenomics, Hot spring, Amylase
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