ISSN: 0973-7510
E-ISSN: 2581-690X
The present study focuses on exploration of an actinomycete from limestone quarries of deccan trap for the production of L-glutaminase. Streptomyces rochei, a promising producer of L-glutaminase was confirmed based on 16S rDNA sequence (DMQ-14: JQ889270) analysis. At optimum level of process parameters (pH-8.0; temperature – 400C ; inoculum size-1×108 spores/ml and agitation-200 rev/min.) a linear increase (9.86±0.025 IU; 12.28±0.010 IU; 13.46±0.015 IU; 15.78±0.02 IU respectively) in the production of L-glutaminase by Streptomyces rochei was achieved. Among the nutritional sources, Starch and L-glutamine at 1.0 % w/v proven to be the best carbon and nitrogen sources for the enhanced production (25.42±0.040 IU and 30.24±0.01 IU respectively) of L-glutaminase. MgSO4, 7H2O (0.05% w/v) proved to be the most suitable metal ion for further increase (31.55±0.020 IU) in the production of L-glutaminase. Thus, with all the optimized conditions, the maximum production of L-glutaminase was 31.55±0.020 IU.
L-glutaminase, Actinomycetes, Limestone quarry, Submerged system, Optimization, 16S rDNA
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