ISSN: 0973-7510
E-ISSN: 2581-690X
Three different agricultural residues were evaluated as basic substrates for the production of biological control agent (BCA) Bacillus subtilis strain B579, by solid-state fermentation (SSF). Among them, wheat bran (WB) was shown to be the most suitable substrate, and an initial moisture content of 57% was determined to be optimal for SSF of strain B579. Moreover, employing the Plackett-Burman and Box-Benhnken experimental design models, nine different supplements were investigated to maximize the cell yield. Results demonstrated that a relatively maximal cell yield of 2.12±0.03×1011 CFU/g was achievable utilizing WB as the basic medium supplemented with beef extract (21.3 g kg-1), cornmeal (42.6 g kg-1), and soluble starch (23.8 g kg-1).
Agricultural residues, Bacillus subtilis B579, Biological Control agent, Design-Expert, Solid-State Fermentation
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