ISSN: 0973-7510
E-ISSN: 2581-690X
Twenty six Rhizobium strains isolated from root nodules of Sesbania sesban (L.) Merr. were studied for their ability to produce bacteriocins. Among them, only seven strains have the ability to produce bacteriocins after 48 hours of growth. The highest activity was shown by SSR-7. The bacteriocin of SSR-7 was optimized by cultural and nutritional conditions. The strain showed highest activity, when tryptone yeast extract medium was supplemented with glucose, galactose and peptone. The pH range for maximum bacteriocin activity was 6.0-7.0. The partially purified bacteriocin was found to be sensitive to proteases and insensitive to DNase and RNase. The activity remained at 80 °C for 5 min. SDS-PAGE analysis of bacteriocin showed the presence of 30 kDa protein.
Bacteriocin production, Rhizobium sp., Sesbania sesban, SDS-PAGE
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