In the present research, bioflocculant was produced from Bacillus subtilis using molecular methods. The structural and functional features of the bioflocculant were determined that it consisted of 64% carbohydrate and 18% protein. Production medium for bioflocculant mainly comprised Sucrose 16 g; Peptone 2 g; MgSO4·7H2O 0.3 g were statistically optimized to increase the bioflocculant production. They were further characterized by Field emission scanning electron microscopy (FE-SEM), and Fourier-transform infrared spectroscopy (FTIR).Therefore, they could be considered as a suitable method for treating municipal wastewater based on high flocculating rate.
Bioflocculant, Wastewater, RSM, Kaolin, Treatment, COD, BOD.
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