A cellulolytic bacterial species APS-8 was isolated from the soils of Visakhapatnam region. This strain is characterized as Gram positive Streptobacillus species. The strain grew well in medium containing Carboxymethylcellulose (CMC), producing maximum enzyme at 37°C and pH 8.0. The activity of extra cellular enzyme was found to be high at temperature 50°C and at pH 5.0. Metal ions like MgSO4, ZnSO4 and FeCl3 enhanced the enzyme activity whereas MnSO4 inhibited the enzyme activity under same assay conditions.
Cellulase, Congo red, Carboxymethylcellulose (CMC), Carboxymethylcellulase (CMCase) assay, APS-8
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