Prevalence of Helicobacter pylori cagA Genotype Among Dyspeptic Patients in Southern Thailand

ISSN: 0973-7510

E-ISSN: 2581-690X

Prevalence of Helicobacter pylori cagA Genotype Among Dyspeptic Patients in Southern Thailand

Sueptrakool Wisessombat¹ and Chatruthai Meethai²

¹School of Allied Health Sciences and Public Health, Walailak University, Tha Sala, Nakhon Si Thammarat Province, Thailand.

²Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla Province, Thailand.

J Pure Appl Microbiol. 2014;8(5):3551-3554

Received: 02/06/2014 | Accepted: 15/07/2014 | Published: 31/10/2014

Abstract

To investigate the prevalence of H. pylori infection in dyspepsia patients and it relation to virulence factor cagA gene. In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex PCR. Multiplex PCR detected three genes of 16S rRNA, cagA, and ureC. H. pylori was detected in 14 gastric biopsies (13%). Significantly higher numbers of female were infected. Furthermore, cagA gene was found in all H. pylori–positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 ^oC was able to effectively amplify specific products. The results conûrmed that high prevalence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand.

Keywords

Helicobacter pylori, Multiplex PCR, Dyspepsia, and cagA gene

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