Possible Negative Regulatory Effects of Antibiotic Production in a Hetrologous System by Sare_4854, a Novel Member of Streptomyces Antibiotic Regulatory Protein Family (SARP) from a Marine Microorganism Salinispora arenicola CNH643 DSM 44819

ISSN: 0973-7510

E-ISSN: 2581-690X

Possible Negative Regulatory Effects of Antibiotic Production in a Hetrologous System by Sare_4854, a Novel Member of Streptomyces Antibiotic Regulatory Protein Family (SARP) from a Marine Microorganism Salinispora arenicola CNH643 DSM 44819

Xiao Shu¹#, Wei Zhang¹#, Jinlong Yu¹#, Shaowen Wu¹#, Yanling Ma¹, Minzhe Zhu¹, Sisi Xia¹, Shichao Xia¹, Hao Zhang¹, Aiying Li^1,2 and Chao Qi¹*

¹Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Huazhong Normal University, Wuhan 430079, China.

²School of Life Sciences, State Key Laboratory of Microbial Technology, Shandong University, Jinan – 250 100, China.

J Pure Appl Microbiol. 2014;8(2):1447-1452

Received: 09/01/2014 | Accepted: 18/03/2014 | Published: 31/04/2014

Abstract

Streptomyces antibiotic regulatory proteins (SARP) normally play positive regulatory roles during streptomyces antibiotic biosynthesis. sare_4854, a gene in Salinispora arenicola CNH643 DSM 44819 was proposed to encode a SARP. Here, sare_4854 was expressed in a heterologous system for functional characterization. However, the antibiotic production in the resultant strain was repressed significantly. Bioinfomatic analysis showed that Sare_4854 has an additional nucleoside triphosphate hydrolases (NTPase) domain at the C-terminus, besides a SARP-like domain (DBD: DNA binding domain and BTAD: bacterial transcriptional activation domain) at the N-terminus conserved in all SARPs. The possible mechanism of Sare_4854 in antibiotic synthesis in this abnormal phenomenon was discussed here.

Keywords

SARP, Sare_4854, antibiotic synthesis, Streptomyces

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