The most common problem associated with pollution of groundwater is due to the mixing of sewage resulting in the presence of pathogenic organisms and bacteria in groundwater. Bacteria are considered to be present every where in most of the biotic and abiotic components of atmosphere including under ground strata.  Natural commodities such as air, water, soil and raw food items, essential for life, are usually contaminated with variety of bacterial species and some of which are pathogenic to human beings and other animals. Eight sampling station of Upper Lake were chosen of the year 2011 for the microbiological studies. The results reveal that the water of Upper Lake(Bhopal) is polluted in some stations and requires suitable measures to check it.