ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
K. Nithya1, V. Shanmugaiah1 , N. Balasubramanian2 and S. Gomathinayagam3
1Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, Madurai – 625 021, Tamil Nadu, India.
2Department of Immunology, School of Biological Sciences, Madurai Kamaraj University, Madurai – 625 021, Tamil Nadu, India.
3Faculty of Agriculture and Forestry, University of Guyana, Berbice Campus, Tain, Guyana, South America
J Pure Appl Microbiol, 2019, 13 (3): 1307-1315 | Article Number: 5711
https://doi.org/10.22207/JPAM.13.3.02 | © The Author(s). 2019
Received: 03/06/2019 | Accepted: 15/07/2019 | Published: 24/09/2019
Abstract

In recent days, antibiotic producing fluorescent pseudomonads (FPs) has been used as a bioorganic tool for the control of sheath blight disease of rice.  Combined application of antagonistic microorganism showed that significant bio control activity and enhances plant growth by induced systemic resistance (ISR). The present study, we carryout  morphological, physiological and biochemical analysis  and then identified, the selected isolate VSMKU2 is Pseudomonas sp. Maximum  level of phenylalanine ammonia lyase (PAL) was quantified  in the treatment  of Pseudomonas sp VSMKU2 + R. solani  on the 7th day (97.50 nmol trans-cinnamic acid/min/g). Similarly, the cell free culture filtrate of VSMKU2 challenged with R. solani demonstrated lower level of PAL activity on 7th day (91.76 nmol trans-cinnamic acid/min/g) compared to control. Peroxidase (PO) and polyphenoloxidase (PPO) gave higher activity in Pseudomonas sp VSMKU2 challenged with R. solani on 7th day (0.94 and 0.95 unit/min/g of proteinrespectively) but 14th and 21st day after challenged inoculation of R. solani had been reduced (0.92, 0.75 and 0.82, 0.65 unit/min/g of protein) compared to control. The total phenol content activity was significantly increased with Pseudomonas sp VSMKU2 (148.27 µg catechol/mg/g of protein) and cell free culture filtrate of VSMKU2 (137 µg catechol/mg/g of protein) treated in rice seedlings on 7th day after challenged inoculation of R. solani compared to control. The results obtained in the current study imply to Pseudomonas sp VSMKU2 was able to rise defence response, thereby contribute resistance to sheath blight disease.

Keywords

Pseudomonas sp VSMKU2, R. solani, Rice seedlings, defence related enzymes.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.