EthOH/Water extract of fenugreek ground seeds was assayed to determine their antimicrobial activity against six bacterial strains Staphylococcus aureus, Bacillus cereus as Gram positive bacterium. Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Salmonella typhi as Gram negative bacterium. Fenugreek extract showed antifungal potential and the magnitude of their inhibitory effects was species dependent. The results of phytochemical screening of fenugreek seeds extract revealed the presence of various chemical compounds such as alkaloids, saponins, flavonoids, phenols, glycosides, Anthocyanin, betacyanin, and steroids. Results showed that the growth of S. aureus and S. typhi was inhibited at a MIC value of 2mg/ml followed by E. coli, P. vulgaris and K. pneumonia, while Ps. aeruginosa showed highest MIC value of 2mg/ml. The study findings provide supportive evidence for the use of Trigonella foenum in traditional medicines and it could be an important source of biologically active compounds useful for developing better new antibacterial drugs.