https://dx.doi.org/10.22207/JPAM.13.2.12 | © The Author(s). 2019
Different loading of Ag nanoparticles (2 and 6%) modified TiO2 synthesized by employing polymeric template; comprises of polyvinyl alcohol and polyethylene glycol (Tev), were utilized to react hydrothermally (433 K for 48 h) with functionalized SWCNT and MWCNT. Several characterization techniques, including UV-visible diffuse reflectance spectroscopy, X-ray diffraction, HRTEM-ASED, and N2 sorptiometry were utilized for obtaining information about surface texturing, morphology, optical properties and crystalline phases. The synthesized photocatalysts were tested for their antibacterial activity against Escherichia coli (Gram negative bacterium) and Staphylococcus aureus (Gram positive bacterium) through agar well diffusion method under visible light irradiation (λ > 450 nm, 60 mWcm-2). It has been revealed that the photocatalyst TevAg6-SWCNT exhibited the maximum lethal action thereby inhibiting the growth of bacteria as compared to rest of the catalysts; this was due to increase in surface area value with delay in electrons and holes recombination as well as the diameter of Ago nanoparticles which was reduced to 3 nm. It has been evidenced that the nanocomposites attack mechanism on while disinfecting bacteria indicates the formation of O2•− and ·OH radicals, those played significant role in inactivation of cellular process. The TEM images revealed the role of Ag+ ions, and the presence of TevAg6-SWCNT were thoroughly studied and correlated to identify the disinfection mechanisms induced through irradiation of visible light.
Ag/TiO2-CNTs nanocomposites, Bacterial disinfection; DNA damage; Surface active species, morphology change.
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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.