ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Salah Abdalrahim1, Abdel Naser A. Zohri2, Manal Khider3, Adel M. Kamal El-Dean4, Hussein H. Abulreesh5 , Iqbal Ahmad6 and Khaled Elbanna5,7
1Sugar Technology Research Institute (STRI), Assiut University, Assiut 71516, Egypt.
2Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt.
3Department of Dairy Science, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
4Department of Chemistry, Faculty of Science, Assiut University, Assiut, Egypt.
5Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
6Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, India.
7Depatment of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum 63514, Egypt.
J Pure Appl Microbiol, 2019, 13 (3): 1349-1362 | Article Number: 5769
https://doi.org/10.22207/JPAM.13.3.06 | © The Author(s). 2019
Received: 20/06/2019 | Accepted: 08/08/2019 | Published: 29/09/2019
Abstract

Exopolysaccharides (EPS) particularly, from Lactic acid bacteria have received increasing attention in food, medical, and pharmaceutical applications. The present work aims to isolate, characterize and identify exopoly saccharide-producing bacteria from fermented fruits and vegetables and dairy products. A total of 55 isolates were isolated from fermented fruits, vegetables, and dairy products depending on the mucoid appearance of the colonies. Based on total EPS production, the most promising nine strains were selected, phenotypically and genotypically characterized. They were facultative anaerobe, arranged in pairs/chains (cocco bacillus), oxidase, and catalase-negative, non-spore forming and non-motile Gram-positive bacteria. All the strains were capable of growing at optimum pH between 5-7, tolerate to NaCl up to 7% (w/v), growing at 20-37°C with optimum growth at 30°C, no growth was observed at 45°C. In addition they could utilize small range of organic compounds, except isolate S1 was differ from the others by their ability to utilize a varied range of organic compounds. Construction of phylogenetic tree, on the basis of partial 16S rRNA gene sequences indicated that isolate S1 was similar to Leuconostoc citreum with similarity of 91.3%, while, isolates S2 and S3 were similar to Leu. fallax and Leu. mesenteroides with similarity of 99.40 % and 97.73%, respectively. Isolates S4, S5, S7, S8, and S9 were similar to Leu. holzaapfelii with similarity of 98.3, 98.7 and 99.8, 98.5 and 98.1, respectively, while isolate S6 was similar to Leu. lactis with similarity of 97.9%. None of sugars such as lactose, glucose, and fructose except sucrose were support EPS production from these strains. The highest yield of EPS was recorded for isolates S6, S1 and S7 which were 61.90, 61.80 and 60 gl-1, respectively, followed by isolates S4, S9, S5 and S8 which were 58.40, 53.06, 51.61 and 33.53 gl-1, respectively. Although, the lowest yield was observed for the isolates S3 and S2 which were 22.08 and 18.80 g l-1, respectively. Finally, it could be concluded that EPS production from these strains in the current study, considering them to be the alternative choice for enhancing production of EPS with increased yields, with promising realistic importance in food, pharmaceutical, as well as dairy industries.

Keywords

Lactic acid bacteria, Leuconostoc, Exopoly saccharids, Genotypic and Phenotypic characterization, Food, dairy, medical, pharmaceutical applications.

Article Metrics

Article View: 102
PDF Download: 16

Share This Article

Journal Tools

© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.