PCR-Based Subtyping of Blastocystis Isolates from Symptomatic and Asymptomatic Patients in North-West of Iran

ISSN: 0973-7510

E-ISSN: 2581-690X

Abbas Shahbazi¹, Esmail Fallah^2*, Peyman Heydarian¹, Ardavan Ghazanchaei², Majid Khanmohammadi³, and Nasrin Mirsamdi⁴

¹Tabriz Research Center of Infectious and Tropical Diseases.

²Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, Iran.

³Sajjad Private Boarding Clinic, Department of Health and Treatment, Tabriz University of Medical Sciences, Tabriz, Iran.

⁴Department of Parasitology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

J Pure Appl Microbiol. 2013;7(4):2957-2963

Received: 11/03/2013 | Accepted: 29/05/2013 | Published: 30/12/2013

Abstract

The pathogenic potential of Blastocystis hominis is still controversial. To find the pathogenicity of this organism determining the genotypic differences among symptomatic and asymptomatic isolates can be useful. To determine genetic diversity of Blastocystis hominis in North West of Iran, 57 isolates from 34 asymptomatic healthy individuals and 23 symptomatic patients were genotyped by polymerase chain reaction using seven pairs of known subtype specific sequence tagged site (STS) primers. Out of 57 isolates, ST3 as predominant (50.87%) followed by ST1 (40.35%) and ST2 (8.77%) were recognized. By comparing genotype of Blastocystis isolates among asymptomatic and symptomatic group, we found that in asymptomatic group subtype 3 was most dominant (29/34) and all patients in symptomatic group classify as subtype 1 (23/23).

Keywords

Blastocystis hominis, PCR, Genotype, Iran

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