ISSN: 0973-7510
E-ISSN: 2581-690X
Conventional methods such as microscopy and culture lack the sensitivity and are time consuming.PCR provides good rate of positive results and better turnaround time than culture (days versus weeks) and smear examination. The present study was to shows the sensitivity and time taken for PCR verses positive culture in the diagnosis of tuberculosis .Molecular diagnosis of tuberculosis by PCR has a great potential to improve the clinicians ability to diagnose tuberculosis, so that early treatment to patients can prevent further spread. This study has demonstrated that MTB PCR assay is rapid, sensitive and highly specific as compared to commonly used conventional techniques. In addition, rapidity of the test allows quick implementation of treatment regimen.
Mycobacterium tuberculosis, PCR, Nested PCR
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