Gujuluva Hari Dinesh1,2, Karthik Sundaram3, Kulanthaisamy Mohanrasu1,2, Ramu Satheesh Murugan1,2, Puthamohan Vinayaga Moorthi4Tondi Rajan Angelin Swetha2, Gopal Selvakumar5 and Alagarsamy Arun2*

 1Department of Energy Science, Alagappa University, Karaikudi, India.
2Department of Microbiology, Alagappa University, Karaikudi, India.
3Department of Microbiology, PSG Arts College, Coimbatore, India.
4Department of Human Genetics and Molecular Biology, Bharathiyar University, Coimbatore, India.
5Department of Microbiology, DDE, Alagappa University, Karaikudi, India.


The bio hydrogen (H2) production by anaerobic digestion of industrial waste is beneficial one due to the availability of proteins and carbohydrates as potential substrate for biological H2 production. An anaerobic fermentative route is a promising method of bio-hydrogenation. The microbial isolates from various industrial wastes (dairy, sugar and food) were assessed for their potential bio H2 production. The selected individual isolates (F1 – Bacillus subtilis and A3 –Bacillus subtilis) and their cocultures were used for the optimization of bio H­2 production utilizing various concentration of biscuit industry waste as substrate at various pH conditions. The mixed consortium which displayed the higher bio H2 production was selected for the detailed analysis of the 3L fermentation studies using 90% Organic Loading Rate (OLR) substrate at pH 6.5. Significantly higher Hydrogen Yield (HY) of 0.87 mol H2/mol glucose on 16th day of incubation was observed.

Keywords: Food waste, substrate, pH, Bio hydrogen, Fermentation, Bacillus subtilis.