Optimization of the Biosynthesis of the Macrolide Antibiotic WA52 Produced by the Alkaliphilic Isolate Nocardiopsis dassonvillei WA52

ISSN: 0973-7510

E-ISSN: 2581-690X

Optimization of the Biosynthesis of the Macrolide Antibiotic WA52 Produced by the Alkaliphilic Isolate Nocardiopsis dassonvillei WA52

Wael N. Hozzein^1,2 , Mohamed Ibrahim A. Ali³ and Maged S. Ahmed²

¹Bioproducts Research Chair (BRC), Zoology Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.

²Department of Botany, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.

³Department of Botany, Faculty of Science, Cairo University, Giza, Egypt.

J Pure Appl Microbiol. 2013;7(4):2551-2558

Received: 28/09/2013 | Accepted: 10/12/2013 | Published: 30/12/2013

Abstract

The effects of some physical and nutritional parameters were studied for the optimum production of a macrolide antibiotic, designated WA52-A, that was produced in the fermentation culture of the alkaliphilic strain Nocardiopsis dassonvillei WA52. The effects of incubation period, initial pH and incubation temperature were evaluated. The results revealed that the maximum antibiotic production was achieved after 5 days incubation at 30ºC with an initial pH of 9. Subsequently, the effects of the nutritional requirements, carbon source, nitrogen source and mineral salts, were investigated. The results indicated that the maximum antibacterial activity was obtained using a fermentation medium with the following composition (g/100ml): Glucose, 2.5; NaNO₃, 0.2; K₂HPO₄, 0.12; MgSO₄, 0.05; KCl, 0.06; and FeSO₄, 0.008.

Keywords

Optimization, antibiotic, alkaliphilic, Nocardiopsis dassonvillei WA52

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