Optimization of Medium Composition for Antibacterial Substance Production by Aspergillus niger xj using Response Surface Methodology

ISSN: 0973-7510

E-ISSN: 2581-690X

Optimization of Medium Composition for Antibacterial Substance Production by Aspergillus niger xj using Response Surface Methodology

Zhu Li¹ , Song Liu¹, Yuan Song², Wu-Juan Liu¹, Xue Chen³, Qiang Wang¹, Yong-Yi Ge¹, Miao-Xiao Shi⁴ and Yi Jin⁵

¹College of Life Sciences, Guizhou University, Guiyang 550025, China.

²School of Marine Science and Technology in Harbin Institute of Technology at Weihai,
Weihai 264200, China.

³Bijie Tobacco Company of Guizhou Province, Bijie 551700, China.

⁴College of Anshun, Guiyang 550005, China.

⁵College of Mathematics and Information Technology, Guiyang University, Guiyang 550005, China.

J Pure Appl Microbiol. 2014;8(2):1635-1643

Received: 11/06/2013 | Accepted: 21/08/2013 | Published: 31/04/2014

Abstract

To investigate the optimal medium composition for antibacterial substance production by Aspergillus niger xj in liquid-state fermentation (LSF). The disc diffusion method was used to assay the production. Statistical methodologies, including the Plackett-Burman design (PBD) and the central composite design (CCD), were employed to investigate the individual crucial component of the medium that significantly affected the production. The optimum values of the critical components for the maximum antibacterial substance production were obtained as follows: x₁(MgSO₄)=-0.3417 (0.2982 g/L), x₂(Tween-80)=-0.28878 (0.242245 ml/L) and the predicted diameter of the inhibitory zone value was 27.39847 mm. Using the optimized condition, the diameter of inhibitory zone reached 27.74±3.07 mm. By using PBD and CCD, we determined the optimal composition for antibacterial substance production by Aspergillus niger xj in LSF.

Keywords

Antibacterial substance, Optimization of medium composition, Plackett-Burman design (PBD), Response surface methodology (RSM), Aspergillus niger xj

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