ISSN: 0973-7510
E-ISSN: 2581-690X
Infectious hematopoietic necrosis (IHN), viral hemorrhagic septicemia (VHS) and infectious pancreatic necrosis (IPN) are important viral diseases of salmonids especially rainbow trout leading to mass mortality among cultured fish. The aim of this study was molecular detection of IHNV, IPNV and VHSV in eyed egg, fries and broodstock of rainbow trout and comparison of their abundance among hatcheries in the western part of Iran. In this study, 300 eyed eggs, 150 fry and 6 broodstocks were studied in 15 hatcheries. All samples were examined by Reverse Transcriptase Polymerase Chain Reaction assay. The frequency of IHNV, IPNV and VHSV were 37.28, 46.96 and 8.19 percent, respectively as 20.89% of the eggs, 54.44% of fries and 17.14% of the broodstocks found to be infected by one of the viruses. The present study revealed that examined fish, both fry and broodstock, were simultaneously infected by all three kind viruses which is very important epidemiologically.
IHN, IPN, VHS, rainbow trout, RT-PCR
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