ISSN: 0973-7510

E-ISSN: 2581-690X

Elsayed E. Hafez1, Emad el-Dein H. Wasfy2, Ahmed H.M. Ramadan3 and M. A. Abdel-Gayed3
1Plant Protection and Biomolecular Diagnosis Department, Arid Land Research Institute, City for Scientific Research and Technology Applications, Egypt.
2Plant Pathology Department, Faculty of Agriculture, Alexandria University, Egypt.
3Onion & Garlic Diseases Research Department, Plant Pathology Research Institute, Agriculture Research Center, Giza, Egypt.
J Pure Appl Microbiol. 2013;7(Spl. Edn.: November):79-91
© The Author(s). 2013
Received: 15/08/2013 | Accepted: 20/10/2013 | Published: 30/11/2013
Abstract

Botrytis aclada and other Botrytis spp. can cause neck rot, a storage disease and blasting diseases on onions. Botrytis byssoidea is investigated and recorded for the first time in Egypt on onion plants associated with seeds and bulb neck rot. Specific primers (BA2f/BA1r) were designed based on genome of Botrytis spp. associated with neck rot and blasting of onions. The partial sequences of the amplified PCR products and the constructed phylogenetic tree distinguished the examined Botrytis isolates into four groups: Botrytis aclada types AI and AII (B. allii); B. byssoidea; and B. cinerea. Results revealed that the major dominant species associated with onion neck rot and blasting was type AI (B. aclada). Whenever, Botrytis byssoidea was not investigated and recorded before on onion plants associated with seeds and bulb neck rot in Egypt. Specificity of the designed primers confirm that only Botrytis spp. can amplify a product of approximately 413 bp but no amplified products were obtained with the other tested genera and species isolated from infected and/or healthy tissues of onion bulbs

Keywords

Neck Rot, Blasting Diseases of Onion, Botrytis spp. and Molecular Identification by PCR

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