ISSN: 0973-7510
E-ISSN: 2581-690X
The objective of the present study was to identify the cellulose degrading Trichoderma viride. Dilution plating was used to quantify the propagule numbers of Trichoderma, denaturing gradient gel electrophoresis (DGGE) and DNA sequence analysis were used to identify Trichoderma species. Trichoderma viride was identified based on the microscopic observations by Lactophenol cotton blue and scanning electron microscopy.
It was ultimately confirmed based on DGGE and DNA sequence analysis and finally concluded by BLAST analysis by constructing a phylogenetic tree.
Trichoderma viride, Dilution plating, 18s RNA, BLAST
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