Rana A.Jawad, Ihsan M. Sulbi, Yasser J. Jameel and
Hayder A. Muhammid

College of Veterinary Medicine / University of Kerbala, Karbala, Iraq.

Abstract

The aim of this study was to choose the optimal PCR technique with specific designing primer design of cystic fluid of ​Echinococcus granulosus hydatid cyst in cattle have slaughtered in kerbala abattoirs / kerbala governorate, the techniques have allowed to reduce the time consumption ,Nucleotide sequences of​ Echinococcus granulosus in Iraq cattle was obtained from NCBI websites with other researchers concerned in this topic, numerous visceral organs Livers , lungs and liver with lung suspected have hydatid cysts were isolated from 35 cow؛s slaughter, In PCR the highest number of positive results have 22(62.8%) were detected as ​Echinococcus granulosus hydatid cyst, on the other organs, the parasite isolation ratio was calculated from internal organs ​the percentage of hydatid cysts were significant increases as 12 (63.6%), 6 (27.2%)and 2(18.1%) for Liver, lung and liver with lung, respectively​.

Keywords:Echinococcus granulosus​ ,​hydatid cyst​ in cow and PCR.