ISSN: 0973-7510

E-ISSN: 2581-690X

Samy Selim1,2
1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,
Aljouf University, Sakaka, P.O. 2014, Saudi Arabia.
2Microbiology and Botany Department, Faculty of Science, Suez Canal University,
Ismailia, P.O.Box 41522, Egypt.
J. Pure Appl. Microbiol., 2016, 10 (3): 1965-1969
© The Author(s). 2016
Received: 11/07/2016 | Accepted: 29/09/2016 | Published: 30/09/2016
Abstract

This study will evaluate Thermoactinomyces vulgaris HSB4 (KR698305) thermophilic microbial isolate for production of thermostable xylanase isolated from water spring in Aljouf, Saudi Arabia. Culture parameters for xylanase production by T. vulgaris were optimized. The enzyme was concentrated by spinning through a centriplus, centrifugal ultrafiltration Millipore membrane with a total yield of 25%. The relative molecular mass of this xylanase determined by SDS-PAGE was ranging from 67-43 KDa. This extracellular xylanase therefore could be defined as thermostable with distinct properties that make the enzyme applicable for different industrial purposes.

Keywords

Thermophilic, Thermoactinomyces vulgaris HSB4, Xylanase.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.