Pyricularia grisea is pathogenic to more than 50 graminaceous hosts including food security crops such as rice, wheat, finger millet, pearl millet and foxtail millet .The fungus Pyricularia grisea is the causal agent of rice blast. Blast is the principal disease of rice because of its wide distribution. AFLP analysis of Pyricularia grisea isolates with 9 primer pair combinations produced total of 452 reproducible fragments. Out of 452 alleles 102 alleles were polymorphic with an average of 11.33% polymorphism per primer combination. Different primer combinations amplified 37 to 56 alleles  with an average of 50.22 alleles  per primer combination. The number of polymorphic alleles  for each primer combination varied from 06 to 15 polymorphic alleles per primer combination. Polymorphic information content (PIC) values ranged from 0.45 to 0.90. Similarity matrix was generated using software NTSYS-pc with Jaccard’s coefficient of similarity. The pathogen population of each hot spot was found to be diverse in the present investigation. Grouping of isolates from different geographic region in one genotype indicated the movement of pathogen.