Molecular Characterization of Lactobacillus plantarum Isolates of Vegetables and Phyllosphere

ISSN: 0973-7510

E-ISSN: 2581-690X

Molecular Characterization of Lactobacillus plantarum Isolates of Vegetables and Phyllosphere

Y.L. Savitha¹ , S.R. Mulla¹, D.M. Preethi², C. Sandeep¹, V.C. Suvarna³ and C.K. Suresh¹

¹Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, India.

²Department of Plant Pathology, University of Agricultural Sciences, GKVK, Bangalore, India.

³Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bangalore, India.

J Pure Appl Microbiol. 2013;7(4):3201-3206

Received: 08/02/2013 | Accepted: 26/03/2013 | Published: 30/12/2013

Abstract

The present study was conducted to know the biological and molecular variability among the Lactobacillus plantarum isolates. Different vegetables and phyllospheres were screened for Lactobacillus spp. Out of the fourteen isolates; ten were identified as Lactobacillus plantarum based on microscopic observation, biochemical tests and by using species specific primer. RAPD marker analysis was used to study the molecular diversity. Total of 37 bands were scored in RAPD analysis and 29 bands were found to be polymorphic. RAPD analysis depicted that ten isolates formed four clusters. Banding pattern of these isolates distinguished the isolates of different sources.

Keywords

Lactobacillus plantarum, Molecular diversity and RAPD

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