Molecular Characterization and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolated from Alkharj, KSA

ISSN: 0973-7510

E-ISSN: 2581-690X

Molecular Characterization and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus Bloodstream Isolated from Alkharj, KSA

Mounir M. Salem-Bekhit^1,2 , Ibrahim M. Abdel Aziz^3,4, Sherif H. Abd-Alrahman⁵, Ibrahim Alsarra⁶ and Fares Alanazi^1,6

¹Kayyali Chair for Pharmaceutical Industries, King Saud University, P. O. Box 2457, Riyadh, Saudi Arabia.

²Microbiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

³Medicine Department, Salman Bin Abdul Aziz College of Medicine, Al Kharj, Saudi Arabia.

⁴Department of Tropical Medicine, Al Azhar Faculty of Medicine, Cairo, Egypt.

⁵Biomarkers Research Program, Biochemistry Department, College of Science, King Saud University, PO Box, 2455, Riyadh, 11451, Saudi Arabia.

⁶Department of Pharmaceutics, College of Pharmacy, King Saud University, P. O. Box 2457, Riyadh, Saudi Arabia.

J Pure Appl Microbiol. 2014;8(Spl. Edn. 1):239-247

Received: 23/03/2014 | Accepted: 02/05/2014 | Published: 31/05/2014

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired infections in most hospitals worldwide. The aim of this study is to investigate the antimicrobial susceptibility, Panton Valentine Leucocidine (PVL) mecA and toxin genes, agr types and staphylococcal cassette chromosome mec (SCCmec) types of MRSA bloodstream isolates collected from Salman University Hospital, Alkharj, KSA. Antimicrobial susceptibilities were investigated by agar diffusion method; PVL, mecA and toxin genes by polymerase chain reaction (PCR) and SCCmec and agr typing were performed by multiplex PCR. Totally MRSA isolates were susceptible to linezolid and glycopeptide vancomycine. mecA gene was detected in totally of the isolates, on the other hand, PVL positive isolate was not detected. sea was the most frequently (76%) detected enterotoxin gene. SCCmec typing revealed type III in 85% and agr typing revealed type I in 87% of the isolates.

Keywords

Antimicrobial susceptibility, MRSA, Molecular epidemiology, PCR, Toxin gene, mecA, SCCmec

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