In this work, a mixture design was used to investigate the influence of ten carbon sources on the production of two pigments, undecylprodigiosin and actinorhodin, from Streptomyces coelicolor. These carbon sources were glucose, sucrose, fructose, mannose, glycerol, mannitol, lactose, starch, maltose and methylcellulose. The effect of each carbon source on the production of each pigment was investigated separately and as a mixture of two carbon sources. According the mixture design, 3.29 of fructose and 6.66 g/L of sucrose can be used to achieve maximum production of undecylprodigiosin (58.07 mg/L). Whereas, in order to achieve maximum production of actinorhodin (67.9 mg/L), culture was supplemented with 9.8 g/L of glucose. However, if the target was to produce both pigments in the same culture, 7.1 g/L of fructose and 2.81 g/L of sucrose have to be used as carbon sources to produce 36.717 and 51.78 mg/L of undecylprodigiosin and actinorhodin respectively. The accuracy of model of the optimized carbon sources suggested by the mixture design for both pigments was verified in several experiments and results were approximately closed to the predicted values. Moreover, according to mixture design, the most carbon sources that influence production of undecylprodigiosin and actinorhodin were glucose, fructose and sucrose.