The aim of this study was to evaluate the microbiological quality of fruits salad commercially sold in Thanjavur city to reveal the microbial safety and possible health hazard linked with consuming this product. A total of 100 samples, were collected from major fruit stalls and local markets are analyzed for aerobic mesophilic and psychrotropic bacterial counts.  85 samples were analyzed for the presence of yeast and moulds, enumeration of coliforms and the presence of E.coli O157:H7 and Salmonella or Shigella species. Presence of aerobic mesophiles ranged from 2.5 to 5.8 log cfu/g, with the lowest and highest counts observed for pineapple (2.0 log cfu/g) and watermelon (6.8 log cfu/g) respectively. The highest coliforms were observed from grapes and freshcut salads with 70% of the samples contain 5 log cfu/g. Similarly, the maximum counts of yeasts and moulds were detected in pomegranates and grapes. There is no psychrotrophic microorganisms were isolated in this study. Biochemical characterization of bacteria was done using Biolog MicroplateTM culture method and pathogens such as Salmonella were present in a few sample and E.coli O157: H7 or Shigella was not found in any of the samples used for the study. However, the presence of bacteria and yeast in higher range shows that the microbiological quality of fresh cut fruit salad sold in Thanjavur city were not recommended level and vendors should practice Good Manufacturing Practices before selling the products to the consumers.