ISSN: 0973-7510

E-ISSN: 2581-690X

Mohammad Zubair Alam1 , Shamim Ahmad2, Esam I. Azhar3,4, Absarul Haque1, Qamre Alam1 
1King Fahd Medical Research Center, King Abdulaziz University, P. O. Box 80216, Jeddah 21589, Saudi Arabia.
2Microbiology Division, Institute of Ophthalmology, Faculty of Medicine, J.N. Medical College, Aligarh Muslim University, Aligarh 202 002 – India.
3Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdul Aziz University, Jeddah, Saudi Arabia.
4Medical Laboratory Technology Department , Faculty of Applied Medical Sciences,
King Abdulaziz University, Jeddah, Saudi Arabia.
J Pure Appl Microbiol. 2014;8(3):1961-1972
© The Author(s). 2014
Received: 15/11/2013 | Accepted: 12/01/2014 | Published: 31/06/2014
Abstract

In the present work, Staphylococcus gallinarum W-61 was studied for the removal of Cd(II), Ni(II), Cr(VI),   Cr(III) ions from aqueous solution. All experiments were conducted with the dried and non growing biomasses of S. gallinarum W-61 under varying conditions of pH, contact time, and initial concentration of the metal ion. The pH of the solution considerably altered the biosorption capacity of metal ions by the test isolate. Biosorption of Cd and Ni was maximum at pH 6.5, pH 4.5 was found optimum for Cr(III) whereas S. gallinarum W-61 adsorbed Cr(VI) maximum at pH 2.5. The removal of metal ions was conspicuously rapid; most of the total adsorption occurred within 30 min of reaction time. The sorption data was analyzed with the Langmuir and Freundlich isotherm models. The highest Qmax and Kf value was found for the biosorption of Cd(II) with 48.8 mg/g and 6.78 mg/g respectively when the experiment was conducted with the non growing biomass of S. gallinarum W-61. Recovery of metal ions (Cr(VI), Cr(III)  Cd(II) and Ni(II)) through desorption  was found better with the dried biomass compared with the non growing biomass of the isolate. The isolate was further tested for its bioaccumulation potential under actively growing conditions. .The results of bioaccumulation shows that S. gallinarum W-61 has accumulated varying amount of test metals intracellularly.  The isolate could be employed for the removal of heavy metals from spent industrial effluents before discharging it into the environment.

Keywords

Biosorption, Bioaccumulation, Metal, Bioremediation, Adsorption isotherms, Staphylococcus gallinarum

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