Hospital-prepared enteral tube feedings (Home based Intact Polymeric Formula and Standard Commercial Formula) from two intensive care units in a multispeciality tertiary care hospital in India were analyzed for microbial contamination. A total number of 4 samples (2 samples each at the time of preparation and 2hours following preparation) were collected. Colony count and Antibiotic Susceptibility tests for all samples were conducted. At the time of preparation, all samples had detectable colony counts. The concentration of microrganisms in both the samples of enteral feeding solutions was >10² CFU/mL as against the permissible level which is (10² CFU/mL or less) (Jalal, 2003) that increased over 4 hours. Microbial contamination varied between sites. The types of organisms identified in both the formulas were Bacillus species, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. There is a high degree of variability in microbial contamination of enteral feeds. It is concluded from this study, that home based intact polymeric formula need not be a source of bacterial contamination, if the feed preparation is carried out under aseptic conditions with care.  Failing of these would lead to bacterial contamination of the feeds, regardless of the type of feeds used.