Microbial community analysis of Panda (Ailuropoda melanoleuca) intestinal system is instructive to control Panda intestinal disease, evolve Herbivory intestinal system and research degradation flora rich in high efficiency fiber. The massively parallel sequencing technology, 454 pyrosequencing technique, was adopted to probe microbial community on Panda fresh excrement for six months. With dominant phyla belonging to Proteobacteria (14.29% of total bacteria), Firmicutes (51.80%), Bacteroidetes (14.19%), Actinobacteria (5.71%), Synergistetes (0.09%), Spirochaetes (0.37%), TM7 (0.18%), unclassified Bacteria (13.36%). At genera level, unclassified “Porphyromonadaceae”, unclassified Lachnospiraceae, unclassified Clostridiaceae , Clostridium XI, unclassified Clostridiales, Advenella and unclassified Bacteria (relative abundances > 2.0%). Clostridium having cellulose degradation function accounted for about 20% microbial population in panda excrement. The panda is generally from the carnivorous intestinal tract to grazing.
Ailuropoda melanoleuca, 454 pyrosequencing technique, Microbial community, Cellulose decomposition bacteria
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