ISSN: 0973-7510

E-ISSN: 2581-690X

Kondepudi Kanthi Kiran1 and T.S. Chandra2
1Department of Chemistry, Biochemistry Laboratory, Indian Institute of Technology Madras, Chennai – 600 036, India.
2Department of Biotechnology, Indian Institute of Technology Madras, Chennai – 600 036, India.
J Pure Appl Microbiol. 2011;5(2):545-552
© The Author(s). 2011
Received: 28/12/2010 | Accepted: 08/02/2011| Published: 31/10/2011
Abstract

Screening bacteria from wet saline soil collected from a salt manufacturing industry, Chennai led to the isolation of a total of 8 moderately halophilic bacteria. One strain, designated as MHA, an obligate and moderate halophile, capable of hydrolyzing soluble starch and tributyrin and another strain, MHX, capable of hydrolyzing xylan were isolated. MHA showed growth in a broad pH range of 6.5-11.0; temperatures in the range of 25-55oC and NaCl from 1-25% (w/v) and failed to grow in absence of NaCl indicating its obligate halophilic nature. It produced 0.150 U/ml of amylase when grown in presence of 1% soluble starch and 10% NaCl. Activity staining of the extracellular acetone precipitated protein fraction on native polyacrylamide gel electrophoresis (PAGE) indicated the secretion of multiple amylases by the strain MHA.

Keywords

Moderate halophile, MHA, Amylase, Lipase, MHX, Xylanase, Native PAGE

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