ISSN: 0973-7510

E-ISSN: 2581-690X

S. Bundale, D. Begde, N. Nashikkar, P. Mashitha, J. Rudra and A. Upadhyay
Hislop School of Biotechnology, Hislop College, Nagpur, India.
J Pure Appl Microbiol. 2010;4(2):809-814
© The Author(s). 2010
Received: 21/04/2010 | Accepted: 30/05/2010| Published: 31/10/2010
Abstract

Streptomyces are soil dwelling, Gram positive, filamentous bacteria belonging to the phylum Actinobacteria. These are high G+C organisms noted for their characteristic ‘earthy’ odour which results from production of a volatile metabolite, geosmin. Streptomyces are known for their ability to produce antibiotics. In fact, two thirds of the known antibiotics are produced by actinomycetes and 80% of them are made by members of Streptomyces genus.

An actinomycete strain, designated SSB, was isolated from the local soil sample. Initial studies indicated that it was a Streptomycete. It was sent to IMTECH, Chandigarh for further identification. On the basis of morphological, phenotypic and biochemical characteristics, it was identified as Streptomyces torulosus, SSB. The strain SSB was found to produce a reddish pigment on both solid as well as liquid media. The pigment production was best observed in Potato dextrose broth (Hi-media) and hence used for further studies on pigment production. Streptomyces torulosus is not reported earlier to produce such a pigment. The pigment produced by the strain SSB was extracted in ethyl acetate and purified by standard chromatographic techniques. The absorption spectra of the partially purified pigment were determined. It was found to be similar to Actinomycin-D. The partially purified pigment was found to have antibacterial activity against gram positive organisms.

Keywords

Streptomyces torulosus, Actinomycin-D, pigment

Article Metrics

Article View: 852

Share This Article

© The Author(s) 2010. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.