Isolation and purification of membrane-bound Cytochrome c and Cytochrome b from Nocardia asteroides

ISSN: 0973-7510

E-ISSN: 2581-690X

Isolation and purification of membrane-bound Cytochrome c and Cytochrome b from Nocardia asteroides

Muhammad Atif Zia¹ , Muhammad Shahid Nazir², Tariq Ismail³, Ahmed Adnan and Muhammad Hassan Qureshi

¹Biochemistry Laboratory (Chemistry Department), Government College University, Lahore, Pakistan.

²Microbiology Laboratory (Biotechnology Department), Government College University, Lahore, Pakistan.

³Hematology Laboratory (Department of Zoology), Government College University, Lahore, Pakistan.

J Pure Appl Microbiol. 2007;1(2):185-190

Received: 10/09/2007 | Accepted: 09/10/2007 | Published: 30/10/2007

Abstract

In present studies, pathogenic bacterium, Nocardia asteroides was investigated. The growth profile of Nocardia asteroides was studied by optimizing the growth conditions by culturing 20-26 hours with maximum growth. Down-streaming process was accompanied by sonication, differential centrifugation, ultracentrifugation and detergent solubilization techniques. Partially purified membrane-bound proteins were analyzed spectrophotometricaly for membrane-bound protein contents. Reduced membrane spectra and SQR (complex II) activity showed the presence of cytochrome b while pyridine ferrohaemochrome of cytochrome c.

Keywords

Cytochrome b, cytochrome c, respiratory chain, Nocardia asteroides

Download PDF

Article Metrics

Article View: 546

JPAM.1.2.08 (198 downloads)

Share This Article

© The Author(s) 2007. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.