ISSN: 0973-7510

E-ISSN: 2581-690X

P. Sreedhar Reddy and D. Jhansi Rani
Nagarjuna College of Engineering and Technology, Modugurki-village, Venkatagiri Kote-Post, Devanahalli – Taluk, Bangalore – 5621 10, India.
J Pure Appl Microbiol. 2011;5(2):801-806
© The Author(s). 2011
Received: 18/02/2011 | Accepted: 21/04/2011| Published: 31/10/2011
Abstract

α-amylase family enzymes have significant importance in wide area with potential application. a-amylase was isolated from air, cultured under optimum condition and subjected for characterization. Each colony is observed under microscope using lacto phenol cotton blue and three different types of Aspergillus species was found i.e A.niger, A.flavus, A. Oryzae. The optimum pH for the activity of the enzyme in A.flavus and A.niger was 4.5 and in A. oryzae optimum pH was 5.0. The optimum temperature for enzyme production was 30°C for A. flavus and A. oryzae and for A. niger it is 40ºC.

Keywords

A. flavus,. A. oryzae, A. niger, a-amylase,. Koji tray culture

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