The biochemical tests which achieved in this study were including; IMViC to diagnose the pink mucoid colonies on MacConkey which suspected to be Klebsiella and after achieving the test it showed indole negative because it doesn’t have tryptophanase enzyme that showed colorless ring after adding the Kovac’s reagent and the colonies gave negative for methyl red test as its showed yellow color but in voges-Proskauer test its showed positive result after adding the reagents and red colour appeared, in addition to positive result for simmons citrate as the media became blue in color that interprets the usage of citrate and change the media to alkaline pH; for suspected colonies of Staphylococcus aureus which grew on Mannitol salt agar with yellowish golden color, coagulase and catalase test were achieved, in catalase test they gave babbles that mean this colony was positive for this test. In coagulase test it gave positive result as clumping was appear on the slide.

Arthropods convey diverse of infectious agents and held a varied group of local microorganisms include bacteria and fungi (Abraham et al., 2017). Ticks regard as the mainly related vectors of pathogens causing disease in the wild and domestic animals and the cattle tick, Rhipicephalus (Boophilus) microplus, where it is endemic, hinder animals production in both subtropical and tropical parts of the world (Andreotti et al., 2011 ), and the significance of ticks in its ability to spread pathogenic microorganisms (Goddard, 2008) such as Borrelia, Ehrlichia, Francisella Rickettsia, Anaplasma and Babesia (de la Fuente et al., 2008), but only a very few reports the isolation of bacteria from hard ticks that infested animals (Rahman and Rahman, 1980; Amoo et al., 1987; Parola and Raoult, 2001; Vilcinset al., 2009; Heise et al., 2010; Andreotti et al., 2011 and Lalzar et al.,  2012).

The results of the study differed from Rahman and Rahman (1980) who conducted a study in Bangladesh by homogenating Rhipicephalus microplus and isolated Enterobacter, Pseudomonas, and Staphylococcus,. Amoo et al. (1987) used the hemolymph to isolate Staphylococcus aureus from adult females and their eggs. Abraham et al. (2017) observed eight genera including Staphylococcus, Corynebacterium, Acinetobacter, Enterococcus, Lysinibacillus, Pseudomonas, and Rickettsia in Ixodesscapularis nymphs. Andreotti et al. ( 2011) identified bacteria at the spp. level include Staphylococcus aureus, Streptococcus dysgalactiae, Staphylococcus chromogenes, Staphylococcus sciuri, Corynebacterium glutamicum, Serratia marcescens and Finegoldia magna. Staphylococcus aureus observed in adult males and also in gut and eggs of adult female in cattle ticks and Stap aureus are the main genera of the bacterial community in the tick samples. Staph were less than 18% in adult males and eggs, but not in adult female ticks; Klebsiella 1.3% in adult female ticks,  that may be due to the environmental contamination, seasons, and type of food used for feeding the animals lead to make a diversity in distribution of livestock having bacteria (Shahzadet al., 2013) or due to the environmental factors that ticks have manners which facilitates the touch with microorganisms in the surroundings that expands the difficulty of bacterial communities to reside on a tick’s exoskeleton and a definitive mechanism which enables the tick to act as a vector which aid the spread of microbes across the hosts and probably influence environmental aspects (Andreotti et al., 2011).

Also, the form of bacterial types influenced by the environment, where the eggs are deposited (Hendry and Rechav, 1981). Within vectors, microbial communities have an significant roles in the capability of pathogens to persist and colonize and transmitted to their host (Cirimotich et al., 2011 and Weiss and Aksoy, 2011). On the same hand, Lalzar et al. (2012) stated that Rickettsia were found in 26% of the tested ticks and this comprised fewer than 10% of the bacterial isolation in females and up to 50% in males, but the density of Rickettsia was considerably lesser than that of Coxiella spp. that sustain association facultative between the tick and Rickettsia and the dynamics and interface patterns may govern of diverse bacterial physiological characteristics. In addition, intrinsic and extrinsic factors may be sharing the environment variation affect the bacterial communities’ composition of arthropod.

Also, the microbiota of arthropods acts an significant role in pathogen resistance, development, physiology of the host, and nutrition (Belongia, 2002) and some factors clarify the low occurrence or plenty of primary or secondary endo-symbionts such as competition, and increased virulence during vertical transmission (Mira and Moran, 2002). Our conclusion, ticks are a good source of Klebsiella spp. and Staphylococcus aureus with easily isolated pathogenic bacteria in the field and may be have a main role in the transmission of these bacteria to the domestic animals.