ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Debasis Mitra1, Anju Rani2 , Lok Man S. Palni1, Komal Sharma1, Navendra Uniyal1, Akansha Chauhan1, Prabhakar Semwal1 and Poonam Arya2
1Department of Biotechnology,Dehradun – 248 002, Uttarakhand, India.
2Department of Life Sciences, Graphic Era (Deemed to be University),Dehradun – 248 002, Uttarakhand, India.
J Pure Appl Microbiol, 2019, 13 (3): 1509-1515 | Article Number: 5672
https://doi.org/10.22207/JPAM.13.3.22 | © The Author(s). 2019
Received: 10/05/2019 | Accepted: 22/06/2019 | Published: 06/08/2019
Abstract

The Himalayan region is conferred by diverse wealth in term of flora and fauna. Among Himalayan plant biodiversity, Saussurea obvallata (common name Brahma Kamal) has an immense spiritual and medicinal significance. Owing to high demand, this herb is on the verge of extinction. Numerous studies have been carried out for preservation and in vitro regeneration of Saussurea obvallata. In view of above, this is the first study  which aims to isolate and identify rhizospheric fungi associated with this plant. Soil samples were collected from three locations in Kedarnath valley and total 34 fungal isolates were isolated. Out of 34, three fungal isolates i.e. MaHaD1, MaHaD2 and MaHaD3 were selected for morphological and molecular characterization. The 16S rDNA sequencing identified MaHaD1, MaHaD2 and MaHaD3 as Phanerochaete chrysosporium, Trichoderma longibrachiatum and Aspergillus fumigatus respectively. The presence of these three dominant fungi in rhizospheric region seems  to be results of climatic conditions and plant physiology. Results indicate that the presence of fungal microflora may play a vital role in survival and proliferation of  Saussurea obvallata plant by providing tolerance and resistance to abiotic stress and fungal/nematode pathogens respectively.

Keywords

Saussurea obvallata, Aspergillus fumigatus, Phanerochaete chrysosporium, Trichoderma longibrachiatum, Rhizospheric soil, rDNA-ITS Sequencing

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.