Sixty-seven pure cultures of halophilic bacteria and yeasts were isolated from Thane creek water, soil, and other materials. High salinities (15% NaCl) were maintained during all phases of the isolation procedure to avoid possible destruction of obligate halophiles. Four of the cultures, all yeasts, proved to be obligate halophiles. Three are species of Candida and one of Torulopsis. They grew in the range of 10% to 20 % salinity and the optimum salinity was 15 %. They died rapidly when exposed to lower saline conditions between 0-5%.
Invertase, halophilic bacteria, halotolerant yeast
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