This study investigated whether the fruit-peel-degrading (FPD) enzyme synthesized by the tropical Rhizobium species CWP G34B in the presence of some fruit peels was formed intracellularly or extracellularly. The Rhizobium spp. CWP G34B was grown in a minimal medium supplemented with 1% (w/v) pectin and peels of apparently healthy mature fruits: pineapple, orange, plantain, banana, pawpaw and mango separately. Dinitrosalicylic acid and Biuret reagent methods were used to quantify glucose and protein in the grown culture and the FPD enzyme activity was based on the amounts of the substances produced by the bacterium. Intracellular enzyme activity ranged between 0.01 mg.ml-1 glucose per mg.ml-1 protein (plantain peel) and 0.05 mg.ml-1 glucose per mg.ml-1 protein (pawpaw and banana peels). The extracellular enzyme activity ranged between 0.75 mg.ml-1 glucose per mg.ml-1 protein (banana peel) and 0.86 mg.ml-1 glucose per mg.ml-1 protein (pineapple peel). The total FPD enzyme activity (0.85 mg.ml-1 glucose per mg.ml-1 protein) was highest in pawpaw peel. The study showed that there was no significant difference between the extracellular and total enzyme activities of the FPD enzyme indicating that the FPD enzyme was majorly produced extracellularly.
Fruit-peel-degrading enzyme activity, Intracellular and Extracellular synthesis
© The Author(s) 2012. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.