https://dx.doi.org/10.22207/JPAM.13.2.08 | © The Author(s). 2019
Salmonella is a gram-negative bacillus of the family Enterobacteraceae, is a pathogen of importance in public health. In Ecuador there is little information regarding this microorganism. So that the objective of this work was to determine the incidence of Salmonella spp. in the different animal species and meat products in through a documentary review. After the documentary study, have been registered a total of 1686 cases of salmonellosis in Ecuador, with a greater presence in the provinces of the Sierra (927 cases), followed by the provinces of the coast (729 cases) and finally the Amazon region with 30 cases. At the provincial level, the highest number of cases of salmonellosis occurs in the province of Pichincha with 805 cases, while, the highest incidence values it is in the province of Sto. Domingo with 85% of incidence. In relation to the level of incidence, the coastal region presents higher average percentage value (38.34%), the most part due to the presence of the pathogen in food, followed by the sierra (27.57), and Amazon region (22.23), however, in the Island region (Galapagos) no quantitative data was registered.
Incidence, Salmonella, Ecuador, animal species, meat products.
The objective of this work was: to determine the incidence of the Salmonella spp. in the different animal species and meat products in Ecuador through the literature review in the period 2009-2019. Salmonella is a Gram-negative bacillus, belongs to the Enterobacteraceae family. Most of the serovarieties that have been isolated from human and from warm-blooded animals belong to the enteric subspecies (subspecies I) and usually, their name is related to the geographical location where they were isolated for the first time (Grigar et al., 2017; Scallan et al., 2011).
There are more than 2.500 serovars or serotypes of Salmonella, the great majority belongs to the subspecies I, here find Salmonella enteritidis and Salmonella typhimurium, which are the most frequent in our environment (Ellermeier and Slauch, 2006).
Epidemiology of Salmonellosis
According by Gyles et al. (2010), from the epidemiological point of view Salmonella spp. it can be classified into three groups:
- Those that do not have a preference for any special host, so infect both human and animals. Most of the serovars responsible for salmonellosis are found in this group.
- Those that infect only human: Salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi C and that are transmitted directly or indirectly from one person to another.
- Those adapted to a host in different animal species, such as sbortusovis in sheep; S. abortusequi in equines and S. gallinarum in birds.
Its wide distribution worldwide and other characteristics that make it resistant has caused a pandemic of Salmonella enteritidis, which first appeared in the eighties and is attributed to the contamination of eggs (Rodrigue et al., 1990). The number of Salmonella infections related to this serotype has been increasing over time. In 1995, there was 36% of Salmonella enteritidis in the world, compared to 65% in 2002 (Herikstad, et al., 2002).
The program WHO-Global Salmonella Survey confirmed that between 2000 and 2004 Salmonella enteric serovar enteritidis, was the most isolated in the world from human matrices; while, in animal samples, others are the Salmonella pathogens commonly founds (Yim et al., 2010).
Salmonella is a bacterium zoonotic that is transmitted through food and requires an adequate temperature for its development between 35 to 37°C. Once in your host organism triggers its toxicity that causes damage to the intestinal mucosa and sometimes the condition extends to a blood level.
The clinical picture usually emerges between the first day to the third day after the contamination, generating discomfort in the individual and symptoms such as dysentery, hyperthermia, pain at the nasogastric level, headaches and uncommon vomiting. When the bacteria reach the bloodstream, the individual is at risk of septicemia, osteomyelitis and meningitis (De Cesare, 2018).
Develoiping Incidence of Salmonella spp. Present in the Costa or Litoral Region of Ecuador
In a study in the province of Esmeraldas on the prevalence of Salmonella in poultry farms, none of the two farms surveyed tested positive for the Salmonella prevalence test (Casart et al., 2018).
According to the research of Galiano et al. (2011), in the data of the National Directorate of Epidemiological Surveillance (DNVE) of the Ministry of Public Health (MSP). It has been reported that in the province of Los Rios, there were 47 cases of infections due to Salmonella spp. probably due to the ingestion of contaminated meat products, of which 39 cases were found in the city of Babahoyo.
In this province, 25 cases of salmone-llosis have been evidenced in meat products that are contaminated during the process of raising transporting the animals, as considered by Rodriguez Merchan and Silva (2017). So also, Sanchez Chugchilon (2016), in their research where the authors detected higher Salmonella incidence during the process of slaughtering chicken skin before chilling with 85% incidence and 50% during the blind process in transport, said pathogen showed resistance to Quinolones.
According to Rodriguez Merchan and Silva (2017), in the province of Manabi after microbiological analysis detected 15 cases of pigs infected with Salmonella, which had white spots in the lungs, liver and intestines. On the other hand, in another study carried out in a processing plant in the city of Manta by Lvia and Trujillo (2013), the authors randomly selected 50 samples of fresh gutted prickly pear and 25 contact surface samples, 150 pinchagua samples in the transport stage and 25 contact surface samples taken from the handlers in the fish market stage; of the total samples analyzed, 4 positive results were detected in the presence of Salmonella spp. on the contact surfaces.
Taxonomic classification of the Salmonella genus
|Le Minor||1985||Salmonella bongori||Gastrointestinal|
|Borman||1957||Salmonella choleraesuis subsp. arizonae||Feces|
|Le Minor||1985||Salmonella choleraesuis subsp. bongori||Feces|
|Smith||1894||Salmonella choleraesuis subsp. choleraesuis||Feces|
|Le Minor||1985||Salmonella choleraesuis subsp. diarizonae||Feces|
|Le Minor||1985||Salmonella choleraesuis subsp. houtenae||Feces|
|Le Minor||1987||Salmonella choleraesuis subsp. indica||Feces|
|Le Minor||1985||Salmonella choleraesuis subsp. salamae||Feces|
|Kauffmann and Edwards||1952||Salmonella enterica||Feces|
|Borman||1957||Salmonella entérica subsp. arizonae||Gastrointestinal|
|Le Minor||1985||Salmonella entérica subsp. bongori||Gastrointestinal|
|Le Minor||1985||Salmonella entérica subsp. diarizonae||Gastrointestinal|
|Le Minor and Popoff||1987||Salmonella entérica subsp. entérica||Gastrointestinal|
|Le Minor and Popoff||1987||Salmonella entérica subsp. houtenae||Gastrointestinal|
|Le Minor and Popoff||1987||Salmonella entérica subsp. indica||Gastrointestinal|
|Le Minor and Popoff||1987||Salmonella enterica subsp. salamae||Gastrointestinal|
|Castellani and Chalmers||1919||Salmonella enteriditis||Gastrointestinal|
|Ezaki||2000||Salmonella paratyphi||Bowels and bloodstream|
|Warren and Scott||1930||Salmonella typhi||Feces|
|Castellani and Chalmers||1919||Salmonella typhimurium||Bowels|
In this province studies were found on the prevalence of Salmonella in meat products in Planning Area 4, where Santa Elena was included. The results showed that, in total, 518 cases were reported for the last morbidity profile in 2016 (Rodríguez Merchan and Silva, 2017).
In the province of Guayas the incidence of Salmonella is very wide since 120 cases of salmonellosis were found according to the research developed by Cedeño (2015), mostly present in poultry, domestic animals, sheep, bovines, among others, are usually the main origin of the contagion of this disease because they are the entity that these bacteria use as a reservoir. On the other hand, in another study carried out by Casart et al. (2018), registered an incidence in poultry farms by 11.1%. Also, in another study conducted by Plaza (2013), where he performed a microbiological analysis on cheeses that are sold in the city of Guayaquil, evidenced the presence of Salmonella spp. in 13.71% of the cheeses analyzed (8/51).
Loayza (2011), carried out a study in the municipal market of the city of Piñas in the province of Oro, where they detected the presence of Salmonella in bovine meat by 66.66%. On the other hand, in the study carried out by Casart et al. (2018), detected Salmonella in 16.70% of the samples analyzed.
Incidence of Salmonella spp. Present in the Sierra or Interandina Region
In a study developed by Campoverde (2014), in Tulcan province of Carchi, where 17 stalls selling artisan sausages were evaluated, and the presence of Salmonella 30.6% were identified in chorizo and 25% in black pudding. In contrast to the results obtained in commercial brands of chorizo where the results were negative for Salmonella.
In a study developed by Sanchez (2016), in a total of 299 samples collected of which: 177 corresponded to raw materials, 10 to finished food, 17 to papers of transport of chickens of 1 day, 51 to farms, 4 to blind, 20 to skin of chickens before and 20 after of the chiller. Resulting in 60 positive samples for Salmonella after molecular analysis.
On the other hand, in the study by Casart et al. (2018), 42.9% of the samples analyzed were positive for Salmonella.
In 2011, 805 cases of salmonellosis were reported in the province of Pichincha, without informing the origin (Direccion Provincial de Salud de Pichincha, 2012). On the other hand, Villagomez et al. (2015), carried out a field work in the poultry slaughtering industrial plant, located in the province of Pichincha, in the sector of Calderon belonging to the Quito. At the end of the sampling, of the 25 skin pools processed and analyzed during this investigation, 20 (80%) were positive for Salmonella spp. In the same way in the investigation, on fecal content of poultry slaughtered in industrial slaughterhouses in Pichincha has reported isolates of Salmonella spp. in 8.45% (n = 142) (Villagomez, 2015). So also, in the study by Casart et al. (2018), obtained a prevalence of Salmonella in 10% of the samples analyzed.
In another study conducted by Vinueza-Burgos et al. (2016), indicated that 15.9% of the batches in the province of Pichincha are Salmonella positive.
Toaquiza (2017), conducted a study in 9 farms in the province of Cotopaxi in 2015, where it was confirmed that there were no outbreaks of salmonellosis.
In addition, according to the OIE, in its 2014 World Animal Health Information System, a total number of 278.444 birds were reported to have outbreaks of Avian Infectious Laryngo-tracheitis and Avian Mycoplasmosis in January and February, as detailed in Table 2.
Cases Susceptible to Avian Infectious Laryngotracheitis in 2014
According to Rodriguez (2013), in his research, they selected 450 chicken eggs, distributed in 150 samples from 50 poultry farms. Of the total samples processed and analyzed, a prevalence of Salmonella enteritidis of 1.3% (2/150) was found. These samples were confirmed by biochemical tests and serotyping. So also, in the study by Casart et al. (2018), obtained a prevalence of Salmonella in 18.2% of the samples analyzed.
In a study conducted by Jara (2016), he investigates the presence of microorganisms in the ground meat sold in the city of Riobamba, detecting for Salmonella presence / 25 g.
An investigation was carried out to determine the incidence of Salmonella types in chicken eggs sold in the markets of Guaranda, Chimbo and San Miguel, 297 samples were analyzed in 99 eggs, being examined both in shell and content (yolk-clara), it was determined that the incidence of Salmonella types in Creole chicken eggs is low (Solano-Buñay, 2015). On the other hand, our research group carried out a study of susceptibility to natural extracts in 32 Salmonella isolates obtained from 100 consumer types of meat (beef, pork and chicken) in the city of Guaranda (Bayas-Morejon et al., 2018).
An investigation was carried out in the community of Oñacapac, where the inhabitants are 100% indigenous, in the Province of Loja, canton Saraguro. The objective of the research work was to determine the causes of mortality in guinea pigs, for which observations were made of the characteristic symptomatology present in the guinea pigs and the microbiological analysis, obtaining a prevalence of 35% (Guaman, 2014). So also, in the study conducted by Casart et al. (2018), obtained a prevalence of Salmonella in 50% of the samples analyzed.
According to Vargas (2016), the main source of infection or transmission of salmone-llosis is found in meat products derived from pigs, since 10 cases of salmonellosis were detected in pigs. This result shows that sales of pork are an important reservoir of this bacterium. and other products of porcine origin should be taken into account as a potential source of Salmonella infection. The results are mainly 43.1% of the total sales of pigs where it was used in the ELISA techniques that are commonly used for the serological diagnosis of porcine salmonellosis.
Of some studies conduced on foods of meat products in the province of Azuay, the presence of Salmonella has not been detected (Paucar and Tenecora, 2013)
Incidence of Salmonella spp. Present in the Amazon Region
No published data have been registered
In the canton Pablo VI, a study was carried out on 10 farms producing milk in the area, in which it was determined that 8 of them were infected with Salmonella, through the tests taken on the milk, a very acute degree of Salmonella was determined in each one of the farms putting the milk-producing area in quarantine and under the protection of treatments for animals (Pelaez, 2009).
In the research carried out by Tena (2015), of the samples obtained in a free trade show, 75% resulted in a negative presence of Salmonella while 25% turned out to be positive out of a total of 12 samples taken. Concluding that the main pork focusses of infection for salmonellosis. So also, in the study by Casart et al. (2018), obtained a prevalence of Salmonella in 33.3% of the samples analyzed.
In a study conducted by Egas (2018), Salmonella was identified in 15.3% (n = 22/144) of the samples analyzed. The only serotype identified was S. Infantis (n = 5).
No published data have been registered
In the canton Pangui, a case of the loss of an establishment of broiler birds was detected where Salmonella spp. contamination had occurred in balanced feed, infecting the net population, resulting in the disease known as typhoid. The Agrocalidad department performed serological tests on the feed and on a batch of animals to determine the infection taken and the reaction of the batch to the same resulting in the involuntary slaughter of the animals in the growth stage.
Incidence Salmonella spp. Present in Galapagos
Aquatic turtles or sea turtles and iguanas are frequently carriers of Salmonella, a bacterium that is lodged in the digestive tract of these animals and can be transmitted to humans with the danger that they will contract the disease of salmonellosis Franco et al. (2011).
Analysis of Data
According to the data investigated, have been registered a total of 1686 cases of salmonellosis in Ecuador, with a greater presence in the provinces of the Sierra (927 cases, followed by the provinces of the coast (729 cases) and finally the Amazon region with 30 cases.
At the provincial level, it can be seen that the highest number of cases of salmonellosis occurs in the province of Pichincha, while in the case of average incidence values it is in the province of Sto. Domingo, Table 4.
Cases and Incidence of Salmonella genus for provinces in Ecuador
|Sto Domingo||25||85.00||2009; 2010|
|Guayas||120||12.35||2015; 2017; 2018|
|El Oro||0||41.00||2011; 2018|
|Pichincha||805||28.36||2012; 2015; 2016; 2018|
|Bolívar||0||35.67||2014; 2015; 2018|
In relation to the level of incidence, the coastal region presents higher average percentage value the most part due to the presence of the pathogen in food, followed by the Sierra, and Amazon region, however, in the Island region (Galapagos) no quantitative data was registered. (Table 3).
Summary of Incidence media values of Salmonella spp. in Ecuador by Regions
The figure below shows the level of incidence by year of Salmonella in meat products
The figure shows that there is a higher incidence of Salmonella in relation to the number of studies conducted were in the years 2009, 2011 and 2018 with average data that exceed 40% of samples recorded.
With more than 1600 registered cases in Ecuador, where the Costa region presents the highest average for the presence of Salmonella and is generally related to food contamination, we can consider that environmental and sanitary conditions (overcrowding of animals in farm), may influence direct on the pathogen incidence.
We would like to express our heartfelt thanks to Professor Dr. Riveliño Ramón-Curay for providing information for the development of this revision work, Thanks to the State University of Bolivar, and to the debt exchange program Ecuador – Spain, for the received support.
Conflict Of Interest
The authors declare that there is no conflict of interest.
FB, AT, IA, PP drafted the manuscript. AT, IA and PP gathered information from the literature about data from the provinces of the coast, sierra and the amazon región respectively. FB compiled information from the literature, and designed the figures and tables. EM and RR supervised and reviewed the manuscript. CZ designed and did the final formatting of the figures and tables.
All datasets generated or analyzed during this study are included in the manuscript and/or the Supplementary Files.
This article does not contain any studies with human participants or animals performed by any of the authors.
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