Infection remains major complication and cause of morbidity in neutropenic cancer patients. A total of 211 clinical samples were collected from 102 patients suffering from different types of malignancies and developed neutropenia. Over all 67(31.75%) samples were positive for bacterial growth. Of there 19 positive were from blood, 32 that of urine, 09 of pus, 05 sputum, one each from stool and throat swab. A total of 70 bacterial isolates belonged to seven different species were obtained from these samples. Out of these 52.86% were gram negative bacilli and 47.14% were gram positive cocci. E.coli was the predominant organism among patients and accounted for 19 (27.14%) in all samples. In the blood highest infective organism was coagulase negative Staphylococcus and accounted for 08(42.10%). High degree of resistance observed in gram negative pathogens. E.coli 19(100%) showed resistance against cefaclor, cefuroxime and amoxicillin plus clavulanate. Eight strains of Klebsiella pneumoniae showed resistance against ceftazidime 8 (73%), cefixime 7 (64%) and to ofloxacin 8(73%). Three strains (21%) of Staphylococcus aureus showed resistant to methicillin. Streptococcus pyogenes showed sensitivity to imipenem and erythromycin. Half of Enterococcus faecalis were resistant to vancomycin. Continuous monitoring of bacterial shift and use of appropriate antibiotic agents reduced morbidity and prolonged survival of neutropenic cancer patients.