ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Samy Selim1,2 , Mohamed Abdel Aziz2, Saher El-Alfay2 and Hanan Zakaria3
1Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, P.O. 2014, Saudi Arabia.
2Department of Botany, Microbiology Section, Faculty of Science, Suez Canal University, Ismailia, Egypt.
3General Health Insurance Authority Hospital, Port Said, Egypt.
J Pure Appl Microbiol, 2019, 13 (3): 1697-1702 | Article Number: 5822
https://doi.org/10.22207/JPAM.13.3.44 | © The Author(s). 2019
Received: 02/08/2019 | Accepted: 08/09/2019 | Published: 24/09/2019
Abstract

This study investigated the prevalence, risk factors and antimicrobial resistance of Staphylococci and E. coli isolated from diabetic urinary tract infection patients. Urine samples collected from 694 cases admitted to the General Health Insurance Authority Hospital in Port Said City, Egypt.  A total of 302 bacterial strains were isolated from 292 urine samples with glucosuria and infection positive. The prevalence of the S. saprophyticus, S. aureus, E. coli and other species isolates from the urine samples are 54.7%, 34%, 7.6% and 3.7%, respectively. The antibacterial resistance profile of the isolated bacteria was investigated against different antibiotics. Most bacterial isolates were more susceptible to oflaxacin antibiotic while resistance to ampicillin. Multiple antibiotic resistance (MAR) was found highest for bacterial isolates obtained from urine samples. S. aureus showed high resistance percentage than other bacterial strains to studied antibiotics. Vancomycin resistance was detected in 23% of all S. aureus isolates. Out of 165 isolates S. saprophyticus, 71% were ג-lactamase producers, while all S. aureus and E. coli isolates were ג-lactamase positive. Also the purpose of this study was to study any relative between the recognized antibiotic resistance of bacteria and the occurrence of plasmids. Molecular sizes of the detected plasmids were 39.306 kbp in S. aureus and 44.640 kbp in E. coli. Plasmid curing in combination with MIC determination revealed that resistance of staphylococci and E. coli isolates was plasmid-mediated linked. The high MAR recognized makes it essential for antibiotic resistance testing to be accompanied prior to antibiotics remedy for diabetic urinary tract infection patients.

Keywords

Antibiotics Resistance, Staphylococci, E. coli, Diabetic Urinary Tract Infection.

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© The Author(s) 2019. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.