ISSN: 0973-7510

E-ISSN: 2581-690X

B.V. Gondhali1 , T.A. More2, R.M. Naik3, K.S. Raghuwanshi4 and P.K. Lokhande5
1Associate Professor of Horticulture, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Dist. Ahmednagar, India.
2Hon. Vice Chancellor, M.P.K.V., Rahuri, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Dist. Ahmednagar, India.
3Professor and Head, Department of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Dist. Ahmednagar, India.
4Associate Professor of Plant Pathology, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Dist. Ahmednagar, India.
5Associate Professor of Biochemistry, Mahatma Phule Krishi Vidyapeeth, Rahuri – 413 722, Dist. Ahmednagar, India.
J. Pure Appl. Microbiol., 2016, 10 (2): 1251-1260
© The Author(s). 2016
Received: 06/12/2015 | Accepted: 17/01/2016 | Published: 30/06/2016
Abstract

In vitro bioefficacies of the different antimicrobial peptides (AMPs) were tested against  three bacteria using paper disc and micro-dilution broth method. Nine different cationic AMPs viz. D4E1, PEP11, ESF1, ESF4, ESF5, ESF6, ESF12, ESF13 and ESF17 exhibiting variable amino acid compositions and charges, were used for the present investigation. Among the various AMPs tested against Xanthomonas axonopodis pv. punicae (Xap), Xanthomonas axonopodis pv. citri (Xacand Xanthomonas axonopodis pv. malvacearum (Xam) ,the peptide D4E1 (net charge + 8) was the most effective AMP against all the three Xanthomonas sp. followed by PEP11, ESF12 and ESF1 having net charge +4.  The peptide ESF13 was not effective against all the three bacteria while ESF4 was not effective against Xap and Xac whereas ESF6 was also not effective against Xac.AntiMicrobial peptides when tested in vitro through micro-dilution broth method showed that the inhibition growth per cent against Xap was greater than 90% (MIC90) for D4E1, PEP11, ESF17, ESF5 and  ESF12. For Xac, D4E1, PEP11, ESF17, ESF5, ESF6, ESF1 and ESF12 showed the inhibition growth percent greater than 90% (MIC90). For Xam,the inhibition growth per cent was seen greater than 90% (MIC90) for D4E1, PEP11, ESF17, ESF1 and ESF12. The most promising peptide D4E1 exhibited better stability against endogenous proteases which recorded only 10.08 % hemolysis at a very high i.e.100 µM concentration.The D4E1 peptides appears to be a promising candidate to be explanted through gene manipulation to control bacterial blight of pomegranate.

Keywords

Anti-Microbial Peptides, Bioefficacy, Xanthomonus sp., Inhibition.

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© The Author(s) 2016. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.