Improvement Lovastatin Production of Aspergillus terreus by pTRLI plasmid Transformation

ISSN: 0973-7510

E-ISSN: 2581-690X

Improvement Lovastatin Production of Aspergillus terreus by pTRLI plasmid Transformation

Dudi Hardianto^1,2, Marlia Singgih Wibowo¹, Amir Musadad¹, Wahono Sumaryono², Juwartina Ida Royani², and Tutus Gusdinar¹

¹School of Pharmacy Technology Institute of Bandung, Indonesia.

²Biotech Center, The Agency for the Assessment and Application of Technology, Indonesia.

J Pure Appl Microbiol. 2012;6(2):609-613

Received: 20/01/2012 | Accepted: 27/02/2012 | Published: 30/06/2012

Abstract

Protoplasts of A. terreus were transformed by pTRLI plasmid containing lovE gene as regulator gene in lovastatin biosynthesis and ptrA gene as pyritiamine resistance gene. Transformats were selected for ability to grow on Czapex-dox agar containing pyrithiamine. Transformats were stable and grew on Czapex-dox agar containing pyrithiamine for 5 generations. A. terreus transformant is tested for lovastatin productivity using medium fermentation. This research showed that copy number of lovE gene in A. terreus increased significantly lovastatin productivity. The lovastatin productivity of A. terreus recombinant was 693 ppm, which is 93% hingher than the parental strain (398 ppm) at 28^oC for 7 days and 200 rpm.

Keywords

Lovastatin, Aspergillus terreus recombinant, Transformation, pTRLI plasmid

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