ISSN: 0973-7510
E-ISSN: 2581-690X
A total of 102 strains of fungi isolated from soil samples in and around Patna. Three of these strains (AB50, AB55, AB109) showing amylase activity were screened for further investigation. Based on morph-physiological characteristics the strain AB55 showing hyper-amylase activity was identified as Aspergillus terreus. The extra cellular amylase production could be detected just after 24 hours under shaking condition and a complete hydrolysis of starch in the medium was observed after 96 hours incubation at 28+20C. Partially purified amylase enzyme showed a maximum activity of 589U/100ml pH 7.0 and at 500C temperature. This strain showed good growth in presence of Fe+2,Ca+2,Mg+2,Co+2 up to a concentration of 5mM. Almost a two fold enhanced activity was observed in the presence of Fe+2,Mg+2and Co+2. However, the ions like Hg+2,Mn+2 and Zn+2 remarkably decreased the activity of amylase enzymes.
Amylase activity, Aspergillus terreus, Metal ions
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