Haematococcus pluvialis can produce a high yield of astaxanthin under stresses, but this induced-biosynthesis is a relatively slow process and low productivity. As blue light can quickly promote astaxanthin accumulation in H. pluvialis, this study further investigated that the impact of blue light illumination from LED (3500 mmol photons m^-2sec^-1) on astaxanthin content, mRNA expression and regulation profiles of eight astaxanthin-synthesis-related carotenogentic genes in H. pluvialis. The results showed that (1) the astaxanthin content was enhanced to 3.03 mg/L in 18 days after blue light induction; (2) the expression levels of carotenoid genes ipi-1, ipi-2, psy, pds, lyc, crtR-b, bkt2 and crtO were up-regulated in different time points during the cultivation; (3) the transcriptional expression of crtR-b, bkt2 and crtO was more efficient than other five genes under blue light induction. It was indicated that genes of crtR-b, bkt2 and crtO might be vital to astaxanthin production in blue light treatment for H. pluvialis. This study suggested that blue-light illumination in H. pluvialis is an effective stimulator to induce astaxanthin production in H. pluvialis.