Immunopathological and Molecular Approaches for the Diagnosis of Salmonellosis in Natural Cases of Bovine Abortion

ISSN: 0973-7510

E-ISSN: 2581-690X

Vishal Mahajan¹ , Harmanjit Singh Banga² and Gursimran Filia¹

¹Department of Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141 004, India.

²Depatment of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141 004, India.

J Pure Appl Microbiol. 2014;8(1):753-756

Received: 28/03/2013 | Accepted: 03/06/2013 | Published: 28/02/2014

Abstract

Rapid and precise diagnosis in natural field cases of bovine abortion warrants use of most sensitive and reliable diagnostic methods. In the present study, histopathological, immuno-histochemical and PCR technique(s) have been applied for diagnosis of abortion cases due to Salmonellosis. Histopathologically, multifocal lymphoid granulomas were observed in liver of aborted fetuse(s) which showed positive immuno-reactivity to the anti-Salmonella polyclonal antibody. PCR for diagnosis of salmonellosis in the aborted bovine fetuses found two positive samples out of the total 113 samples tested. Primers S18/ S19 pair amplified a 159 bp region of the sequence encoding an ompC gene of Salmonella species.

Keywords

Abortion, Immuno-histochemistry, PCR, Salmonellosis

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