Pseudomonas aeruginosa (P. aeruginosa) is the most important, resistant and dangerous organism in burn wound infections in human. Extracellular and virulence factors lead to tissue damage during infection with P. aeruginosa. One important  virulence factor including exotoxin A(ETA)  encoded by the tox A gene.  In this study , 45 wound swabs from burned skin patients admitted to Al-Hilla Teaching Hospital/Babylon, 2013 from different localities in Babylon were collected to study the frequency of P. aeruginosa and human immune response. Polymerase chain traction technique (PCR) was used for detection ETA gene as a virulent factor producing by P. aeruginosa from  burned skin wound infections together with interferon gamma and interleukin 18. The results showed that P. aeruginosa had a frequency of 51.1% among burned skin patients. The bacteriological culture showed that 19 out of 45 (42.2 %) were positive for P. aeruginosa while 23 out of 45 (51.1 %) were positive for P. aeruginosa. Only 17 out of 23 (73.91 %) were ETA producing P. aeruginosa. Also bacteremia occurred only in 9 out of 17 (52.94 %) in ETA producing P. aeruginosa. P. aeruginosa infection was presented to a lesser degree. In addition to that P. aeruginosa that expresses the ETA gene were the most common and toxic pathogens in burned patients and lead to bacteremia and septicemia. The using of PCR technique a rapid and accurate technique might be helpful in combating its toxicity. ETA might contributed to the overall virulent factor.